Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]
Transcription factors with Perturb-seq knockdown data for MRPS27. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPS27 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPS27, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:72,057,600–72,058,552 | 262.2 kb | Distal (>10kb) Multiome | 65 | |
| chr5:72,065,796–72,066,276 | 254.2 kb | Distal (>10kb) Multiome | 107 | |
| chr5:72,106,794–72,109,078 | 211.4 kb | Distal (>10kb) Multiome | 816 | |
| chr5:72,307,892–72,308,896 | 11.8 kb | Distal (>10kb) Multiome | 956 | |
| chr5:72,319,763–72,320,908 | 104 bp | At TSS Multiome | 876 | |
| chr5:72,541,250–72,542,235 | 221.6 kb | Distal (>10kb) Multiome | 116 | |
| chr5:72,556,396–72,557,392 | 236.7 kb | Distal (>10kb) Multiome | 362 |
Genomic view of the MRPS27 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.