Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPL47. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL47 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL47, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:179,322,380–179,323,876 | 281.7 kb | Distal (>10kb) Multiome | 958 | |
| chr3:179,346,338–179,348,224 | 257.0 kb | Distal (>10kb) Multiome | 843 | |
| chr3:179,450,888–179,452,034 | 153.1 kb | Distal (>10kb) Multiome | 580 | |
| chr3:179,504,159–179,505,204 | 100.0 kb | Distal (>10kb) Multiome | 128 | |
| chr3:179,526,783–179,527,418 | 77.5 kb | Distal (>10kb) Multiome | 358 | |
| chr3:179,562,395–179,564,138 | 41.8 kb | Distal (>10kb) Multiome | 866 | |
| chr3:179,604,200–179,605,304 | 97 bp | At TSS Multiome | 786 | |
| chr3:179,608,248–179,609,637 | 4.2 kb | Proximal (<10kb) Multiome | 176 | |
| chr3:179,652,424–179,653,975 | 48.4 kb | Distal (>10kb) Multiome | 805 | |
| chr3:179,868,079–179,869,236 | 264.0 kb | Distal (>10kb) Multiome | 140 |
Genomic view of the MRPL47 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.