Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPL35. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL35 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL35, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:85,921,071–85,922,368 | 277.7 kb | Distal (>10kb) Multiome | 280 | |
| chr2:85,996,436–85,996,920 | 202.7 kb | Distal (>10kb) Multiome | 200 | |
| chr2:86,105,431–86,106,679 | 93.5 kb | Distal (>10kb) Multiome | 923 | |
| chr2:86,172,557–86,173,357 | 26.6 kb | Distal (>10kb) Multiome | 74 | |
| chr2:86,194,668–86,196,003 | 4.1 kb | Proximal (<10kb) Multiome | 1056 | |
| chr2:86,199,308–86,199,778 | at TSS | At TSS | 718 | |
| chr2:86,336,997–86,338,159 | 138.1 kb | Distal (>10kb) Multiome | 267 | |
| chr2:86,368,200–86,368,804 | 169.0 kb | Distal (>10kb) Multiome HiCAR | 227 | |
| chr2:86,440,468–86,442,936 | 241.7 kb | Distal (>10kb) Multiome | 1229 |
Genomic view of the MRPL35 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.