Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene. [provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for MRPL30. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL30 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL30, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:98,935,565–98,937,394 | 244.9 kb | Distal (>10kb) Multiome | 344 | |
| chr2:99,081,586–99,082,682 | 99.0 kb | Distal (>10kb) Multiome | 73 | |
| chr2:99,140,631–99,142,044 | 39.8 kb | Distal (>10kb) Multiome | 976 | |
| chr2:99,154,503–99,155,535 | 26.2 kb | Distal (>10kb) Multiome | 1013 | |
| chr2:99,179,243–99,179,432 | 1.7 kb | Proximal (<10kb) | 88 | |
| chr2:99,180,725–99,181,615 | 15 bp | At TSS Multiome | 1014 | |
| chr2:99,335,843–99,338,098 | 156.3 kb | Distal (>10kb) Multiome | 1129 |
Genomic view of the MRPL30 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.