Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]
Transcription factors with Perturb-seq knockdown data for MRM3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRM3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRM3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:714,554–715,298 | 67.5 kb | Distal (>10kb) Multiome | 798 | |
| chr17:731,870–733,144 | 50.0 kb | Distal (>10kb) Multiome | 602 | |
| chr17:751,539–752,928 | 30.0 kb | Distal (>10kb) Multiome | 891 | |
| chr17:781,922–782,799 | 43 bp | At TSS Multiome | 914 | |
| chr17:875,256–875,758 | 93.1 kb | Distal (>10kb) Multiome | 42 | |
| chr17:927,652–928,423 | 145.6 kb | Distal (>10kb) Multiome | 315 | |
| chr17:933,255–933,971 | 151.3 kb | Distal (>10kb) Multiome | 400 | |
| chr17:951,413–952,178 | 169.4 kb | Distal (>10kb) Multiome | 385 | |
| chr17:964,907–965,931 | 183.2 kb | Distal (>10kb) Multiome HiCAR | 256 | |
| chr17:978,189–980,530 | 197.5 kb | Distal (>10kb) Multiome HiCAR | 639 | |
| chr17:996,479–997,532 | 214.5 kb | Distal (>10kb) Multiome HiCAR | 994 | |
| chr17:1,031,608–1,032,193 | 249.5 kb | Distal (>10kb) Multiome | 54 |
Genomic view of the MRM3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.