This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MLH3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MLH3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MLH3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:74,763,078–74,763,795 | 288.2 kb | Distal (>10kb) Multiome | 808 | |
| chr14:74,879,596–74,880,720 | 171.4 kb | Distal (>10kb) Multiome | 108 | |
| chr14:74,881,522–74,882,475 | 169.6 kb | Distal (>10kb) Multiome | 877 | |
| chr14:74,922,804–74,923,467 | 128.2 kb | Distal (>10kb) Multiome | 458 | |
| chr14:74,955,358–74,956,099 | 95.8 kb | Distal (>10kb) Multiome | 457 | |
| chr14:74,980,538–74,981,185 | 70.6 kb | Distal (>10kb) Multiome | 205 | |
| chr14:75,002,395–75,003,398 | 48.7 kb | Distal (>10kb) Multiome | 767 | |
| chr14:75,050,911–75,051,628 | 104 bp | At TSS Multiome | 749 | |
| chr14:75,063,590–75,064,414 | 12.6 kb | Distal (>10kb) Multiome | 518 | |
| chr14:75,069,008–75,070,183 | 18.1 kb | Distal (>10kb) Multiome | 874 | |
| chr14:75,126,669–75,127,381 | 75.5 kb | Distal (>10kb) Multiome | 888 | |
| chr14:75,176,098–75,177,235 | 125.4 kb | Distal (>10kb) Multiome | 975 | |
| chr14:75,196,367–75,197,128 | 145.2 kb | Distal (>10kb) Multiome | 438 | |
| chr14:75,228,139–75,228,628 | 176.9 kb | Distal (>10kb) Multiome | 65 | |
| chr14:75,229,856–75,230,789 | 179.0 kb | Distal (>10kb) Multiome | 293 | |
| chr14:75,258,653–75,259,760 | 207.7 kb | Distal (>10kb) Multiome | 840 | |
| chr14:75,276,919–75,277,969 | 225.7 kb | Distal (>10kb) Multiome | 731 | |
| chr14:75,278,060–75,280,702 | 227.2 kb | Distal (>10kb) Multiome | 928 | |
| chr14:75,293,606–75,295,425 | 242.8 kb | Distal (>10kb) Multiome | 759 | |
| chr14:75,295,693–75,296,382 | 244.8 kb | Distal (>10kb) Multiome | 471 |
Genomic view of the MLH3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.