This gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Transcription factors with Perturb-seq knockdown data for MKRN2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MKRN2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MKRN2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:12,287,512–12,289,112 | 269.2 kb | Distal (>10kb) Multiome | 592 | |
| chr3:12,444,448–12,445,657 | 112.1 kb | Distal (>10kb) Multiome | 551 | |
| chr3:12,483,888–12,485,008 | 72.6 kb | Distal (>10kb) Multiome | 832 | |
| chr3:12,556,440–12,557,557 | 5 bp | At TSS Multiome | 1025 | |
| chr3:12,662,566–12,663,343 | 105.7 kb | Distal (>10kb) Multiome | 320 | |
| chr3:12,663,393–12,664,764 | 107.2 kb | Distal (>10kb) Multiome | 941 | |
| chr3:12,768,768–12,769,541 | 212.0 kb | Distal (>10kb) Multiome | 69 | |
| chr3:12,796,430–12,797,162 | 239.7 kb | Distal (>10kb) Multiome | 467 | |
| chr3:12,809,737–12,810,783 | 253.3 kb | Distal (>10kb) Multiome | 598 | |
| chr3:12,833,218–12,834,303 | 276.5 kb | Distal (>10kb) Multiome | 191 | |
| chr3:12,841,044–12,841,991 | 284.6 kb | Distal (>10kb) Multiome | 956 |
Genomic view of the MKRN2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.