Abscission, the separation of daughter cells at the end of cytokinesis, is effected by endosomal sorting complexes required for transport III (ESCRT-III). The protein encoded by this gene functions as a homodimer, with the N-termini binding to a subset of ESCRT-III subunits and the C-termini binding to membranes. The encoded protein regulates ESCRT-III activity and is required for proper cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]
Transcription factors with Perturb-seq knockdown data for MITD1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MITD1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MITD1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:98,935,565–98,937,394 | 244.8 kb | Distal (>10kb) Multiome | 344 | |
| chr2:99,081,586–99,082,682 | 98.9 kb | Distal (>10kb) Multiome | 73 | |
| chr2:99,140,631–99,142,044 | 39.7 kb | Distal (>10kb) Multiome | 976 | |
| chr2:99,154,503–99,155,535 | 26.1 kb | Distal (>10kb) Multiome | 1013 | |
| chr2:99,179,243–99,179,432 | 1.6 kb | Proximal (<10kb) | 88 | |
| chr2:99,180,725–99,181,615 | 78 bp | At TSS Multiome | 1014 | |
| chr2:99,335,843–99,338,098 | 156.4 kb | Distal (>10kb) Multiome | 1129 |
Genomic view of the MITD1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.