MICAL1

microtubule associated monooxygenase, calponin and LIM domain containing 1 | DKFZp434B1517, FLJ11937, FLJ21739, MICAL, NICAL

This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Biological processes 39 terms

F-actin monooxygenase activity (GO:0120501)FAD binding (GO:0071949)FAD binding (GO:0071949)NAD(P)H oxidase H2O2-forming activity (GO:0016174)NAD(P)H oxidase H2O2-forming activity (GO:0016174)SH3 domain binding (GO:0017124)actin binding (GO:0003779)actin binding (GO:0003779)actin cytoskeleton (GO:0015629)actin filament binding (GO:0051015)actin filament bundle assembly (GO:0051017)actin filament depolymerization (GO:0030042)actin filament depolymerization (GO:0030042)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoskeleton (GO:0005856)cytoskeleton organization (GO:0007010)cytosol (GO:0005829)cytosol (GO:0005829)endosome membrane (GO:0010008)endosome membrane (GO:0010008)hippocampal mossy fiber expansion (GO:1990026)intercellular bridge (GO:0045171)intermediate filament (GO:0005882)midbody (GO:0030496)midbody (GO:0030496)monooxygenase activity (GO:0004497)negative regulation of apoptotic process (GO:0043066)oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen (GO:0016709)oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen (GO:0016709)plasma membrane (GO:0005886)plasma membrane bounded cell projection (GO:0120025)protein binding (GO:0005515)protein kinase binding (GO:0019901)regulation of regulated secretory pathway (GO:1903305)signal transduction (GO:0007165)small GTPase binding (GO:0031267)sulfur oxidation (GO:0019417)sulfur oxidation (GO:0019417)

Expression (TPM)

TFs regulating MICAL1 0 TFs

Transcription factors with Perturb-seq knockdown data for MICAL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MICAL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to MICAL1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MICAL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr6:109,186,108–109,187,056	269.3 kb	Distal (>10kb) Multiome	39
chr6:109,236,275–109,236,762	219.1 kb	Distal (>10kb) Multiome	258
chr6:109,381,700–109,383,452	73.1 kb	Distal (>10kb) Multiome	1036
chr6:109,440,119–109,441,745	15.0 kb	Distal (>10kb) Multiome HiCAR	863
chr6:109,454,912–109,456,586	175 bp	At TSS Multiome	733
chr6:109,459,229–109,459,417	3.5 kb	Proximal (<10kb)	46
chr6:109,482,359–109,484,058	27.7 kb	Distal (>10kb) Multiome	675
chr6:109,678,985–109,679,628	223.5 kb	Distal (>10kb) Multiome	55
chr6:109,690,198–109,691,786	235.5 kb	Distal (>10kb) Multiome	761

Genome Browser

Genomic view of the MICAL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.