This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for MED22. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED22 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED22, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:133,059,688–133,060,833 | 287.2 kb | Distal (>10kb) Multiome | 222 | |
| chr9:133,120,589–133,121,652 | 226.4 kb | Distal (>10kb) Multiome | 599 | |
| chr9:133,144,161–133,144,920 | 203.1 kb | Distal (>10kb) Multiome | 776 | |
| chr9:133,147,412–133,149,938 | 198.3 kb | Distal (>10kb) Multiome | 979 | |
| chr9:133,163,575–133,164,128 | 183.8 kb | Distal (>10kb) Multiome HiCAR | 308 | |
| chr9:133,238,117–133,239,420 | 109.1 kb | Distal (>10kb) Multiome | 620 | |
| chr9:133,335,737–133,336,702 | 11.5 kb | Distal (>10kb) Multiome HiCAR | 1038 | |
| chr9:133,347,434–133,348,830 | 453 bp | At TSS Multiome | 893 | |
| chr9:133,351,969–133,352,293 | 3.8 kb | Proximal (<10kb) | 435 | |
| chr9:133,356,029–133,357,223 | 8.9 kb | Proximal (<10kb) Multiome | 648 | |
| chr9:133,375,394–133,376,740 | 28.5 kb | Distal (>10kb) Multiome | 834 | |
| chr9:133,417,747–133,418,516 | 70.4 kb | Distal (>10kb) Multiome | 1015 | |
| chr9:133,428,444–133,429,113 | 81.0 kb | Distal (>10kb) Multiome | 255 | |
| chr9:133,429,621–133,430,196 | 82.2 kb | Distal (>10kb) Multiome | 197 | |
| chr9:133,458,883–133,460,984 | 112.2 kb | Distal (>10kb) Multiome | 835 | |
| chr9:133,478,485–133,479,696 | 131.5 kb | Distal (>10kb) Multiome | 653 | |
| chr9:133,534,455–133,535,168 | 187.0 kb | Distal (>10kb) Multiome | 295 | |
| chr9:133,608,924–133,609,730 | 261.7 kb | Distal (>10kb) Multiome | 411 |
Genomic view of the MED22 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.