This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Transcription factors with Perturb-seq knockdown data for MED21. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED21 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED21, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:26,831,973–26,834,292 | 189.2 kb | Distal (>10kb) Multiome | 945 | |
| chr12:26,884,786–26,885,430 | 137.6 kb | Distal (>10kb) Multiome | 412 | |
| chr12:26,937,248–26,939,182 | 84.3 kb | Distal (>10kb) Multiome | 853 | |
| chr12:27,013,686–27,014,988 | 8.2 kb | Proximal (<10kb) Multiome | 864 | |
| chr12:27,017,324–27,017,851 | 4.7 kb | Proximal (<10kb) | 96 | |
| chr12:27,021,899–27,022,924 | 90 bp | At TSS Multiome | 783 | |
| chr12:27,243,875–27,245,004 | 221.7 kb | Distal (>10kb) Multiome | 897 |
Genomic view of the MED21 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.