The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MDC1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MDC1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MDC1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:30,466,230–30,466,713 | 250.7 kb | Distal (>10kb) Multiome | 369 | |
| chr6:30,489,117–30,490,512 | 227.0 kb | Distal (>10kb) Multiome | 750 | |
| chr6:30,516,006–30,516,707 | 200.9 kb | Distal (>10kb) Multiome | 739 | |
| chr6:30,555,407–30,557,765 | 160.6 kb | Distal (>10kb) Multiome | 1161 | |
| chr6:30,570,822–30,571,949 | 145.9 kb | Distal (>10kb) Multiome | 1000 | |
| chr6:30,614,393–30,615,939 | 102.1 kb | Distal (>10kb) Multiome | 837 | |
| chr6:30,616,770–30,618,155 | 99.8 kb | Distal (>10kb) Multiome | 1072 | |
| chr6:30,626,560–30,627,206 | 90.4 kb | Distal (>10kb) Multiome | 802 | |
| chr6:30,646,505–30,648,035 | 70.0 kb | Distal (>10kb) Multiome | 961 | |
| chr6:30,672,571–30,673,266 | 44.3 kb | Distal (>10kb) Multiome | 812 | |
| chr6:30,679,032–30,679,479 | 38.1 kb | Distal (>10kb) Multiome | 603 | |
| chr6:30,686,268–30,688,032 | 30.0 kb | Distal (>10kb) Multiome HiCAR | 801 | |
| chr6:30,690,445–30,691,240 | 26.3 kb | Distal (>10kb) Multiome | 644 | |
| chr6:30,716,986–30,717,874 | 194 bp | At TSS Multiome | 1059 | |
| chr6:30,719,428–30,720,577 | 2.9 kb | Proximal (<10kb) Multiome | 724 | |
| chr6:30,720,820–30,721,086 | 3.5 kb | Proximal (<10kb) | 500 | |
| chr6:30,721,307–30,722,103 | 4.2 kb | Proximal (<10kb) Multiome | 872 | |
| chr6:30,722,280–30,722,892 | 5.0 kb | Proximal (<10kb) | 533 | |
| chr6:30,725,731–30,726,564 | 8.9 kb | Proximal (<10kb) Multiome | 478 | |
| chr6:30,740,996–30,745,094 | 25.5 kb | Distal (>10kb) Multiome | 1233 | |
| chr6:30,763,647–30,765,193 | 47.1 kb | Distal (>10kb) Multiome | 850 | |
| chr6:30,880,638–30,881,295 | 163.7 kb | Distal (>10kb) Multiome | 124 | |
| chr6:30,882,560–30,883,422 | 165.6 kb | Distal (>10kb) Multiome | 308 | |
| chr6:30,883,625–30,885,122 | 167.1 kb | Distal (>10kb) Multiome | 538 | |
| chr6:30,907,770–30,908,494 | 190.8 kb | Distal (>10kb) Multiome | 1005 | |
| chr6:30,913,674–30,914,800 | 196.9 kb | Distal (>10kb) Multiome | 944 | |
| chr6:30,942,475–30,943,087 | 225.5 kb | Distal (>10kb) Multiome | 300 | |
| chr6:30,955,192–30,955,975 | 238.4 kb | Distal (>10kb) Multiome | 524 |
Genomic view of the MDC1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.