Transcription factors with Perturb-seq knockdown data for MCPH1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCPH1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCPH1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:6,559,766–6,561,284 | 147.7 kb | Distal (>10kb) Multiome HiCAR | 551 | |
| chr8:6,562,645–6,563,524 | 145.0 kb | Distal (>10kb) Multiome HiCAR | 199 | |
| chr8:6,699,363–6,700,087 | 8.1 kb | Proximal (<10kb) | 41 | |
| chr8:6,706,385–6,706,763 | 1.4 kb | Proximal (<10kb) | 184 | |
| chr8:6,707,768–6,709,358 | 166 bp | At TSS Multiome | 1010 | |
| chr8:6,800,522–6,801,235 | 92.7 kb | Distal (>10kb) Multiome | 483 | |
| chr8:6,834,068–6,835,901 | 127.3 kb | Distal (>10kb) Multiome | 395 | |
| chr8:6,840,478–6,841,374 | 132.7 kb | Distal (>10kb) Multiome | 350 | |
| chr8:6,863,803–6,864,675 | 155.7 kb | Distal (>10kb) Multiome | 77 |
Genomic view of the MCPH1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.