The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MCM4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:47,737,107–47,739,302 | 222.6 kb | Distal (>10kb) Multiome | 803 | |
| chr8:47,959,857–47,961,766 | 805 bp | At TSS Multiome | 1108 | |
| chr8:48,008,109–48,008,981 | 47.6 kb | Distal (>10kb) Multiome | 921 | |
| chr8:48,136,263–48,137,632 | 176.0 kb | Distal (>10kb) Multiome | 209 | |
| chr8:48,159,677–48,160,187 | 198.9 kb | Distal (>10kb) Multiome | 52 | |
| chr8:48,253,714–48,254,674 | 293.3 kb | Distal (>10kb) Multiome | 248 | |
| chr8:48,921,143–48,922,276 | 960.8 kb | Distal (>10kb) Multiome HiCAR | 612 |
Genomic view of the MCM4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.