The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]
Transcription factors with Perturb-seq knockdown data for MCM3AP. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM3AP upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM3AP, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr21:46,097,574–46,098,972 | 188.2 kb | Distal (>10kb) Multiome | 294 | |
| chr21:46,153,138–46,153,696 | 132.9 kb | Distal (>10kb) Multiome | 515 | |
| chr21:46,184,645–46,185,202 | 101.6 kb | Distal (>10kb) Multiome | 334 | |
| chr21:46,228,249–46,230,022 | 57.3 kb | Distal (>10kb) Multiome | 833 | |
| chr21:46,285,753–46,286,753 | 82 bp | At TSS Multiome | 983 | |
| chr21:46,323,408–46,325,506 | 37.7 kb | Distal (>10kb) Multiome | 952 | |
| chr21:46,457,952–46,459,810 | 172.5 kb | Distal (>10kb) Multiome | 880 |
Genomic view of the MCM3AP locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.