The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
Transcription factors with Perturb-seq knockdown data for MCM2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:127,411,192–127,411,666 | 187.0 kb | Distal (>10kb) Multiome | 109 | |
| chr3:127,455,528–127,456,092 | 142.7 kb | Distal (>10kb) Multiome | 441 | |
| chr3:127,544,147–127,544,731 | 54.0 kb | Distal (>10kb) Multiome | 252 | |
| chr3:127,547,261–127,548,296 | 50.5 kb | Distal (>10kb) Multiome | 460 | |
| chr3:127,589,888–127,591,601 | 7.5 kb | Proximal (<10kb) Multiome | 860 | |
| chr3:127,597,808–127,598,722 | 99 bp | At TSS Multiome | 825 | |
| chr3:127,628,865–127,629,778 | 30.7 kb | Distal (>10kb) Multiome | 643 | |
| chr3:127,672,199–127,673,619 | 74.5 kb | Distal (>10kb) Multiome | 714 | |
| chr3:127,732,310–127,732,974 | 134.1 kb | Distal (>10kb) Multiome | 234 |
Genomic view of the MCM2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.