The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MCM10. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM10 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM10, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:13,000,971–13,001,949 | 160.2 kb | Distal (>10kb) Multiome | 227 | |
| chr10:13,099,052–13,100,750 | 62.4 kb | Distal (>10kb) Multiome | 675 | |
| chr10:13,120,751–13,121,780 | 40.3 kb | Distal (>10kb) Multiome | 155 | |
| chr10:13,161,059–13,162,040 | 96 bp | At TSS Multiome | 746 | |
| chr10:13,299,293–13,300,384 | 138.4 kb | Distal (>10kb) Multiome HiCAR | 850 | |
| chr10:13,302,089–13,302,853 | 140.8 kb | Distal (>10kb) Multiome HiCAR | 807 | |
| chr10:13,347,195–13,348,918 | 186.6 kb | Distal (>10kb) Multiome | 753 | |
| chr10:13,349,410–13,350,393 | 188.4 kb | Distal (>10kb) Multiome | 432 | |
| chr10:13,351,365–13,351,877 | 190.0 kb | Distal (>10kb) Multiome | 105 | |
| chr10:13,440,173–13,440,755 | 279.0 kb | Distal (>10kb) Multiome | 414 |
Genomic view of the MCM10 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.