MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for MARCHF5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MARCHF5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MARCHF5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:92,045,582–92,046,227 | 245.3 kb | Distal (>10kb) Multiome | 132 | |
| chr10:92,087,615–92,088,099 | 203.2 kb | Distal (>10kb) Multiome HiCAR | 401 | |
| chr10:92,290,248–92,291,760 | 58 bp | At TSS Multiome | 955 | |
| chr10:92,350,016–92,350,962 | 59.4 kb | Distal (>10kb) Multiome | 107 | |
| chr10:92,573,390–92,574,819 | 283.0 kb | Distal (>10kb) Multiome | 974 |
Genomic view of the MARCHF5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.