The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MAPK14. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAPK14 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAPK14, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:35,727,435–35,728,457 | 299.8 kb | Distal (>10kb) Multiome | 812 | |
| chr6:35,731,025–35,732,630 | 296.1 kb | Distal (>10kb) Multiome | 1000 | |
| chr6:35,764,004–35,765,052 | 263.2 kb | Distal (>10kb) Multiome | 254 | |
| chr6:35,776,412–35,776,992 | 251.1 kb | Distal (>10kb) Multiome | 410 | |
| chr6:35,804,967–35,805,624 | 222.5 kb | Distal (>10kb) Multiome | 815 | |
| chr6:35,920,112–35,921,529 | 106.7 kb | Distal (>10kb) Multiome | 965 | |
| chr6:35,931,065–35,931,702 | 96.5 kb | Distal (>10kb) Multiome | 77 | |
| chr6:36,024,369–36,024,660 | 3.1 kb | Proximal (<10kb) | 84 | |
| chr6:36,027,295–36,028,609 | 102 bp | At TSS Multiome | 1031 | |
| chr6:36,130,184–36,131,324 | 102.7 kb | Distal (>10kb) Multiome | 615 | |
| chr6:36,196,013–36,197,672 | 168.7 kb | Distal (>10kb) Multiome | 825 | |
| chr6:36,284,908–36,285,433 | 257.4 kb | Distal (>10kb) Multiome | 104 |
Genomic view of the MAPK14 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.