This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
Transcription factors with Perturb-seq knockdown data for MAP3K8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAP3K8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAP3K8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:30,160,563–30,161,110 | 273.2 kb | Distal (>10kb) Multiome | 189 | |
| chr10:30,348,845–30,349,638 | 84.8 kb | Distal (>10kb) Multiome | 582 | |
| chr10:30,359,548–30,360,380 | 74.0 kb | Distal (>10kb) Multiome | 215 | |
| chr10:30,392,543–30,393,007 | 41.3 kb | Distal (>10kb) Multiome | 314 | |
| chr10:30,432,849–30,435,403 | 29 bp | At TSS Multiome | 1011 | |
| chr10:30,436,312–30,436,542 | 2.3 kb | Proximal (<10kb) | 219 | |
| chr10:30,538,047–30,538,841 | 104.3 kb | Distal (>10kb) Multiome HiCAR | 355 | |
| chr10:30,552,960–30,554,430 | 120.1 kb | Distal (>10kb) Multiome | 591 | |
| chr10:30,557,884–30,558,900 | 124.5 kb | Distal (>10kb) Multiome | 267 |
Genomic view of the MAP3K8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.