This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MAP3K3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAP3K3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAP3K3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:63,445,554–63,447,581 | 176.2 kb | Distal (>10kb) Multiome | 896 | |
| chr17:63,476,359–63,478,095 | 145.2 kb | Distal (>10kb) Multiome | 520 | |
| chr17:63,478,783–63,479,370 | 143.3 kb | Distal (>10kb) Multiome | 419 | |
| chr17:63,522,589–63,523,803 | 99.3 kb | Distal (>10kb) Multiome | 705 | |
| chr17:63,549,660–63,551,443 | 72.1 kb | Distal (>10kb) Multiome | 1037 | |
| chr17:63,600,715–63,601,423 | 21.5 kb | Distal (>10kb) Multiome | 1122 | |
| chr17:63,612,337–63,612,552 | 9.9 kb | Proximal (<10kb) | 308 | |
| chr17:63,621,512–63,623,359 | 385 bp | At TSS Multiome | 956 | |
| chr17:63,698,705–63,701,428 | 77.7 kb | Distal (>10kb) Multiome | 663 | |
| chr17:63,741,119–63,742,935 | 119.4 kb | Distal (>10kb) Multiome | 1057 | |
| chr17:63,773,179–63,774,727 | 151.3 kb | Distal (>10kb) Multiome | 1169 | |
| chr17:63,826,794–63,828,024 | 204.9 kb | Distal (>10kb) Multiome | 1008 | |
| chr17:63,841,547–63,843,442 | 220.5 kb | Distal (>10kb) Multiome | 1203 | |
| chr17:63,848,836–63,849,566 | 226.8 kb | Distal (>10kb) Multiome | 658 |
Genomic view of the MAP3K3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.