Transcription factors with Perturb-seq knockdown data for MANEA-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MANEA-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MANEA-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:95,356,616–95,358,322 | 219.8 kb | Distal (>10kb) Multiome | 235 | |
| chr6:95,560,573–95,561,856 | 16.3 kb | Distal (>10kb) Multiome | 217 | |
| chr6:95,573,425–95,573,613 | 3.8 kb | Proximal (<10kb) | 3 | |
| chr6:95,573,739–95,574,065 | 3.4 kb | Proximal (<10kb) | 18 | |
| chr6:95,574,863–95,575,038 | 2.4 kb | Proximal (<10kb) | 133 | |
| chr6:95,576,843–95,578,264 | 99 bp | At TSS Multiome | 823 | |
| chr6:95,802,492–95,803,538 | 225.7 kb | Distal (>10kb) Multiome | 181 | |
| chr6:95,819,126–95,820,464 | 242.4 kb | Distal (>10kb) Multiome | 173 |
Genomic view of the MANEA-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.