N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]
Transcription factors with Perturb-seq knockdown data for MANEA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MANEA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MANEA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:95,356,616–95,358,322 | 219.9 kb | Distal (>10kb) Multiome | 235 | |
| chr6:95,560,573–95,561,856 | 16.4 kb | Distal (>10kb) Multiome | 217 | |
| chr6:95,573,425–95,573,613 | 3.9 kb | Proximal (<10kb) | 3 | |
| chr6:95,573,739–95,574,065 | 3.5 kb | Proximal (<10kb) | 18 | |
| chr6:95,574,863–95,575,038 | 2.5 kb | Proximal (<10kb) | 133 | |
| chr6:95,576,843–95,578,264 | 21 bp | At TSS Multiome | 823 | |
| chr6:95,802,492–95,803,538 | 225.6 kb | Distal (>10kb) Multiome | 181 | |
| chr6:95,819,126–95,820,464 | 242.3 kb | Distal (>10kb) Multiome | 173 |
Genomic view of the MANEA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.