There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for LIMK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LIMK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LIMK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:30,968,406–30,969,906 | 243.2 kb | Distal (>10kb) Multiome | 897 | |
| chr22:31,080,687–31,081,783 | 131.1 kb | Distal (>10kb) Multiome | 636 | |
| chr22:31,084,596–31,085,743 | 127.2 kb | Distal (>10kb) Multiome | 448 | |
| chr22:31,098,574–31,099,237 | 113.4 kb | Distal (>10kb) Multiome | 150 | |
| chr22:31,104,824–31,105,447 | 107.2 kb | Distal (>10kb) Multiome | 187 | |
| chr22:31,107,269–31,107,915 | 104.6 kb | Distal (>10kb) Multiome | 627 | |
| chr22:31,127,262–31,127,766 | 84.7 kb | Distal (>10kb) Multiome | 74 | |
| chr22:31,140,179–31,140,721 | 71.7 kb | Distal (>10kb) Multiome | 244 | |
| chr22:31,160,052–31,160,698 | 52.0 kb | Distal (>10kb) Multiome | 709 | |
| chr22:31,211,835–31,212,727 | 65 bp | At TSS Multiome | 769 | |
| chr22:31,290,525–31,291,372 | 78.6 kb | Distal (>10kb) Multiome | 818 | |
| chr22:31,345,143–31,347,910 | 133.8 kb | Distal (>10kb) Multiome | 954 | |
| chr22:31,362,564–31,363,231 | 150.6 kb | Distal (>10kb) Multiome | 164 | |
| chr22:31,399,166–31,400,036 | 187.2 kb | Distal (>10kb) Multiome | 913 | |
| chr22:31,489,111–31,490,354 | 277.6 kb | Distal (>10kb) Multiome | 819 | |
| chr22:31,496,060–31,496,942 | 284.2 kb | Distal (>10kb) Multiome | 769 |
Genomic view of the LIMK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.