Transcription factors with Perturb-seq knockdown data for LEF1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LEF1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LEF1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:108,160,821–108,161,152 | 6.4 kb | Proximal (<10kb) | 64 | |
| chr4:108,166,123–108,169,630 | at TSS | At TSS | 688 | |
| chr4:108,170,727–108,171,044 | 3.2 kb | Proximal (<10kb) | 120 | |
| chr4:108,171,178–108,173,760 | 3.7 kb | Proximal (<10kb) | 615 |
Genomic view of the LEF1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.