The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for KPNA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KPNA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KPNA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:116,279,028–116,280,325 | 401.4 kb | Distal (>10kb) Multiome HiCAR | 994 | |
| chr6:116,461,655–116,462,775 | 219.0 kb | Distal (>10kb) Multiome | 421 | |
| chr6:116,570,743–116,571,945 | 109.9 kb | Distal (>10kb) Multiome | 1041 | |
| chr6:116,615,808–116,617,150 | 64.9 kb | Distal (>10kb) Multiome | 493 | |
| chr6:116,668,238–116,668,984 | 12.5 kb | Distal (>10kb) Multiome | 746 | |
| chr6:116,680,569–116,682,340 | 74 bp | At TSS Multiome | 965 | |
| chr6:116,764,518–116,766,081 | 84.4 kb | Distal (>10kb) Multiome | 447 | |
| chr6:116,876,238–116,878,022 | 196.0 kb | Distal (>10kb) Multiome | 339 |
Genomic view of the KPNA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.