Transcription factors with Perturb-seq knockdown data for ISL1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ISL1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ISL1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:51,377,141–51,379,761 | 3.6 kb | Proximal (<10kb) | 245 | |
| chr5:51,381,588–51,382,293 | 1.0 kb | Proximal (<10kb) | 89 | |
| chr5:51,382,501–51,384,199 | at TSS | At TSS | 426 | |
| chr5:51,387,200–51,388,228 | 3.9 kb | Proximal (<10kb) | 209 | |
| chr5:51,388,444–51,390,546 | 5.1 kb | Proximal (<10kb) | 237 | |
| chr5:51,390,715–51,391,601 | 7.4 kb | Proximal (<10kb) | 146 | |
| chr5:51,392,697–51,393,166 | 9.4 kb | Proximal (<10kb) | 72 |
Genomic view of the ISL1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.