The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Transcription factors with Perturb-seq knockdown data for IREB2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IREB2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IREB2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:78,148,893–78,150,142 | 288.9 kb | Distal (>10kb) Multiome | 746 | |
| chr15:78,264,436–78,265,112 | 173.5 kb | Distal (>10kb) Multiome | 241 | |
| chr15:78,299,130–78,299,874 | 138.6 kb | Distal (>10kb) Multiome HiCAR | 798 | |
| chr15:78,303,744–78,304,214 | 134.4 kb | Distal (>10kb) Multiome HiCAR | 287 | |
| chr15:78,340,100–78,340,745 | 97.9 kb | Distal (>10kb) Multiome | 194 | |
| chr15:78,348,106–78,348,824 | 89.8 kb | Distal (>10kb) Multiome | 178 | |
| chr15:78,437,554–78,438,897 | 69 bp | At TSS Multiome | 918 | |
| chr15:78,506,965–78,508,087 | 69.3 kb | Distal (>10kb) Multiome | 674 | |
| chr15:78,539,941–78,541,020 | 102.3 kb | Distal (>10kb) Multiome | 773 | |
| chr15:78,564,790–78,566,388 | 127.3 kb | Distal (>10kb) Multiome | 618 | |
| chr15:78,619,950–78,621,461 | 182.0 kb | Distal (>10kb) Multiome | 274 | |
| chr15:78,640,579–78,641,869 | 203.1 kb | Distal (>10kb) Multiome | 406 | |
| chr15:78,671,569–78,672,301 | 233.7 kb | Distal (>10kb) Multiome | 254 |
Genomic view of the IREB2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.