N-methylation of endogenous and xenobiotic compounds is a major method by which they are degraded. This gene encodes an enzyme that N-methylates indoles such as tryptamine. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream MINDY4 (aka FAM188B) gene. In rodents and other mammals such as cetartiodactyla this gene is in the opposite orientation compared to its orientation in human and other primates and this gene appears to have been lost in carnivora and chiroptera. [provided by RefSeq, Jul 2019]
Transcription factors with Perturb-seq knockdown data for INMT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = INMT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of INMT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:30,472,499–30,473,444 | 279.2 kb | Distal (>10kb) Multiome | 123 | |
| chr7:30,478,426–30,479,128 | 273.5 kb | Distal (>10kb) Multiome | 756 | |
| chr7:30,504,202–30,505,455 | 247.2 kb | Distal (>10kb) Multiome | 824 | |
| chr7:30,544,196–30,544,698 | 207.6 kb | Distal (>10kb) Multiome | 40 | |
| chr7:30,594,119–30,595,511 | 157.3 kb | Distal (>10kb) Multiome | 1016 | |
| chr7:30,681,420–30,682,927 | 69.6 kb | Distal (>10kb) Multiome | 319 | |
| chr7:30,699,970–30,700,584 | 51.8 kb | Distal (>10kb) Multiome | 149 | |
| chr7:30,760,943–30,761,477 | 8.8 kb | Proximal (<10kb) | 278 | |
| chr7:30,771,029–30,771,985 | 19.4 kb | Distal (>10kb) Multiome | 536 | |
| chr7:30,938,297–30,939,075 | 186.6 kb | Distal (>10kb) Multiome | 403 | |
| chr7:30,983,471–30,984,223 | 231.7 kb | Distal (>10kb) Multiome | 269 | |
| chr7:31,051,730–31,053,126 | 300.2 kb | Distal (>10kb) Multiome | 203 |
Genomic view of the INMT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.