Predicted to enable protein sequestering activity. Predicted to be involved in several processes, including GCN2-mediated signaling; cellular response to amino acid starvation; and regulation of gene expression. Predicted to act upstream of or within negative regulation of protein phosphorylation. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for IMPACT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IMPACT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IMPACT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:24,138,655–24,139,879 | 287.6 kb | Distal (>10kb) Multiome | 483 | |
| chr18:24,176,686–24,177,648 | 249.5 kb | Distal (>10kb) Multiome | 194 | |
| chr18:24,271,142–24,272,382 | 154.7 kb | Distal (>10kb) Multiome | 485 | |
| chr18:24,368,558–24,369,702 | 57.6 kb | Distal (>10kb) Multiome HiCAR | 140 | |
| chr18:24,397,168–24,398,771 | 28.8 kb | Distal (>10kb) Multiome | 651 | |
| chr18:24,426,310–24,427,225 | 28 bp | At TSS Multiome | 799 |
Genomic view of the IMPACT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.