Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]
Transcription factors with Perturb-seq knockdown data for IL34. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IL34 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IL34, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:70,346,498–70,347,297 | 299.7 kb | Distal (>10kb) Multiome | 945 | |
| chr16:70,380,270–70,382,056 | 265.1 kb | Distal (>10kb) Multiome | 1036 | |
| chr16:70,415,208–70,415,782 | 231.1 kb | Distal (>10kb) Multiome | 229 | |
| chr16:70,427,766–70,429,282 | 218.1 kb | Distal (>10kb) Multiome | 981 | |
| chr16:70,431,040–70,431,529 | 215.4 kb | Distal (>10kb) Multiome | 302 | |
| chr16:70,436,957–70,437,432 | 209.3 kb | Distal (>10kb) Multiome | 457 | |
| chr16:70,438,422–70,439,873 | 207.3 kb | Distal (>10kb) Multiome | 926 | |
| chr16:70,454,276–70,454,976 | 192.0 kb | Distal (>10kb) Multiome | 845 | |
| chr16:70,457,673–70,458,151 | 188.6 kb | Distal (>10kb) Multiome | 38 | |
| chr16:70,523,103–70,524,281 | 122.9 kb | Distal (>10kb) Multiome | 903 | |
| chr16:70,653,817–70,654,034 | 7.3 kb | Proximal (<10kb) | 386 | |
| chr16:70,679,595–70,680,466 | 33.6 kb | Distal (>10kb) Multiome | 216 | |
| chr16:70,685,497–70,686,558 | 39.5 kb | Distal (>10kb) Multiome | 655 | |
| chr16:70,800,702–70,801,519 | 154.6 kb | Distal (>10kb) Multiome | 768 |
Genomic view of the IL34 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.