This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome. [provided by RefSeq, Mar 2017]
Transcription factors with Perturb-seq knockdown data for IFT74. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IFT74 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IFT74, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:26,682,826–26,683,655 | 273.2 kb | Distal (>10kb) Multiome | 130 | |
| chr9:26,891,915–26,893,136 | 63.8 kb | Distal (>10kb) Multiome | 980 | |
| chr9:26,946,640–26,948,029 | 9.1 kb | Proximal (<10kb) Multiome | 852 | |
| chr9:26,955,897–26,957,142 | 36 bp | At TSS Multiome | 646 | |
| chr9:26,974,839–26,976,887 | 19.9 kb | Distal (>10kb) Multiome | 281 |
Genomic view of the IFT74 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.