Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for IARS2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IARS2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IARS2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:219,834,079–219,834,880 | 259.6 kb | Distal (>10kb) Multiome | 136 | |
| chr1:219,839,143–219,840,267 | 254.3 kb | Distal (>10kb) Multiome HiCAR | 246 | |
| chr1:220,045,998–220,046,836 | 47.6 kb | Distal (>10kb) Multiome | 976 | |
| chr1:220,086,269–220,086,448 | 7.7 kb | Proximal (<10kb) | 126 | |
| chr1:220,089,552–220,090,573 | 4.2 kb | Proximal (<10kb) Multiome | 779 | |
| chr1:220,093,676–220,094,911 | 35 bp | At TSS Multiome | 815 | |
| chr1:220,271,709–220,273,089 | 178.3 kb | Distal (>10kb) Multiome | 984 | |
| chr1:220,338,584–220,339,585 | 244.8 kb | Distal (>10kb) Multiome | 180 |
Genomic view of the IARS2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.