This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for HYAL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HYAL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HYAL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:50,088,748–50,089,596 | 232.3 kb | Distal (>10kb) Multiome | 677 | |
| chr3:50,121,279–50,122,037 | 199.5 kb | Distal (>10kb) Multiome | 364 | |
| chr3:50,138,509–50,140,035 | 181.9 kb | Distal (>10kb) Multiome | 198 | |
| chr3:50,154,437–50,155,741 | 166.2 kb | Distal (>10kb) Multiome | 450 | |
| chr3:50,204,913–50,205,545 | 116.0 kb | Distal (>10kb) Multiome | 367 | |
| chr3:50,205,645–50,206,357 | 115.0 kb | Distal (>10kb) Multiome | 347 | |
| chr3:50,226,120–50,228,328 | 95.0 kb | Distal (>10kb) Multiome | 915 | |
| chr3:50,235,551–50,236,824 | 85.2 kb | Distal (>10kb) Multiome | 632 | |
| chr3:50,240,985–50,241,805 | 79.9 kb | Distal (>10kb) Multiome | 137 | |
| chr3:50,245,721–50,247,161 | 74.9 kb | Distal (>10kb) Multiome | 671 | |
| chr3:50,259,568–50,260,922 | 61.0 kb | Distal (>10kb) Multiome | 869 | |
| chr3:50,264,309–50,265,446 | 56.4 kb | Distal (>10kb) Multiome | 568 | |
| chr3:50,275,163–50,276,639 | 44.7 kb | Distal (>10kb) Multiome | 151 | |
| chr3:50,291,628–50,292,796 | 28.7 kb | Distal (>10kb) Multiome | 855 | |
| chr3:50,298,495–50,300,110 | 21.7 kb | Distal (>10kb) Multiome | 872 | |
| chr3:50,303,363–50,303,840 | 17.6 kb | Distal (>10kb) Multiome | 484 | |
| chr3:50,320,603–50,322,739 | 356 bp | At TSS Multiome | 816 | |
| chr3:50,324,456–50,325,020 | 3.5 kb | Proximal (<10kb) Multiome | 392 | |
| chr3:50,327,933–50,328,581 | 7.1 kb | Proximal (<10kb) Multiome | 697 | |
| chr3:50,336,856–50,338,227 | 16.3 kb | Distal (>10kb) Multiome | 952 | |
| chr3:50,340,624–50,341,322 | 19.7 kb | Distal (>10kb) Multiome | 345 | |
| chr3:50,345,443–50,346,042 | 24.5 kb | Distal (>10kb) Multiome | 209 | |
| chr3:50,350,496–50,351,298 | 29.7 kb | Distal (>10kb) Multiome | 902 | |
| chr3:50,358,889–50,360,012 | 38.4 kb | Distal (>10kb) Multiome | 796 | |
| chr3:50,364,552–50,365,867 | 44.1 kb | Distal (>10kb) Multiome | 331 | |
| chr3:50,435,932–50,436,533 | 115.0 kb | Distal (>10kb) Multiome | 248 | |
| chr3:50,502,505–50,504,160 | 182.5 kb | Distal (>10kb) Multiome | 312 | |
| chr3:50,567,311–50,568,123 | 246.5 kb | Distal (>10kb) Multiome | 376 | |
| chr3:50,568,824–50,569,934 | 248.1 kb | Distal (>10kb) Multiome | 901 | |
| chr3:50,611,315–50,612,530 | 290.7 kb | Distal (>10kb) Multiome | 904 | |
| chr3:50,615,997–50,617,867 | 295.9 kb | Distal (>10kb) Multiome | 754 |
Genomic view of the HYAL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.