The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for HUS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HUS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HUS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:47,896,547–47,897,454 | 82.7 kb | Distal (>10kb) Multiome | 134 | |
| chr7:47,969,370–47,969,760 | 9.9 kb | Proximal (<10kb) | 35 | |
| chr7:47,978,959–47,980,059 | 27 bp | At TSS Multiome | 1028 | |
| chr7:47,988,920–47,989,098 | 9.3 kb | Proximal (<10kb) | 26 | |
| chr7:47,989,729–47,990,281 | 10.4 kb | Distal (>10kb) Multiome | 165 | |
| chr7:47,991,664–47,992,347 | 12.4 kb | Distal (>10kb) Multiome | 539 | |
| chr7:48,035,130–48,036,475 | 55.9 kb | Distal (>10kb) Multiome | 452 | |
| chr7:48,038,675–48,039,550 | 59.5 kb | Distal (>10kb) Multiome | 70 | |
| chr7:48,088,257–48,090,514 | 109.6 kb | Distal (>10kb) Multiome | 970 |
Genomic view of the HUS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.