Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. The sulfotransferase domain of this enzyme is highly similar to the same domain of heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1, and these two enzymes sulfate an identical disaccharide. This gene is widely expressed, with the most abundant expression in liver and placenta. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for HS3ST3A1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HS3ST3A1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HS3ST3A1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:13,575,395–13,576,482 | 25.8 kb | Distal (>10kb) Multiome | 251 | |
| chr17:13,594,873–13,595,050 | 6.9 kb | Proximal (<10kb) | 208 | |
| chr17:13,600,452–13,602,595 | 177 bp | At TSS Multiome | 570 | |
| chr17:13,749,684–13,750,484 | 148.1 kb | Distal (>10kb) Multiome | 98 | |
| chr17:14,069,121–14,069,983 | 467.5 kb | Distal (>10kb) Multiome HiCAR | 810 | |
| chr17:14,297,092–14,298,113 | 695.6 kb | Distal (>10kb) Multiome HiCAR | 567 | |
| chr17:14,300,397–14,303,082 | 699.0 kb | Distal (>10kb) Multiome HiCAR | 904 | |
| chr17:14,308,769–14,310,537 | 707.5 kb | Distal (>10kb) Multiome HiCAR | 830 |
Genomic view of the HS3ST3A1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.