This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The protein also acts as a modulator of the antiviral immune response. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Aug 2021]
Transcription factors with Perturb-seq knockdown data for HERC5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HERC5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HERC5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:88,158,357–88,159,406 | 298.1 kb | Distal (>10kb) Multiome | 719 | |
| chr4:88,178,032–88,179,178 | 278.6 kb | Distal (>10kb) Multiome | 219 | |
| chr4:88,283,347–88,285,223 | 172.4 kb | Distal (>10kb) Multiome HiCAR | 864 | |
| chr4:88,378,676–88,379,604 | 78.0 kb | Distal (>10kb) Multiome | 741 | |
| chr4:88,456,441–88,457,951 | 81 bp | At TSS Multiome | 865 | |
| chr4:88,523,195–88,524,307 | 66.7 kb | Distal (>10kb) Multiome | 927 | |
| chr4:88,591,644–88,593,513 | 135.3 kb | Distal (>10kb) Multiome | 832 |
Genomic view of the HERC5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.