Transcription factors with Perturb-seq knockdown data for HDAC2-AS2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HDAC2-AS2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HDAC2-AS2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:113,776,752–113,777,865 | 192.7 kb | Distal (>10kb) Multiome | 198 | |
| chr6:113,854,564–113,856,446 | 114.9 kb | Distal (>10kb) Multiome | 538 | |
| chr6:113,856,568–113,860,608 | 112.7 kb | Distal (>10kb) Multiome | 1105 | |
| chr6:113,969,821–113,972,040 | 159 bp | At TSS Multiome | 1107 | |
| chr6:114,421,138–114,422,029 | 451.5 kb | Distal (>10kb) Multiome HiCAR | 148 |
Genomic view of the HDAC2-AS2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.