Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Transcription factors with Perturb-seq knockdown data for HARS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HARS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HARS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:140,400,395–140,402,925 | 289.8 kb | Distal (>10kb) Multiome | 1129 | |
| chr5:140,546,917–140,548,500 | 143.7 kb | Distal (>10kb) Multiome | 784 | |
| chr5:140,556,971–140,557,959 | 133.9 kb | Distal (>10kb) Multiome | 886 | |
| chr5:140,564,055–140,565,123 | 126.7 kb | Distal (>10kb) Multiome | 827 | |
| chr5:140,639,043–140,640,146 | 51.9 kb | Distal (>10kb) Multiome | 898 | |
| chr5:140,647,175–140,648,159 | 43.6 kb | Distal (>10kb) Multiome | 863 | |
| chr5:140,664,368–140,665,161 | 26.6 kb | Distal (>10kb) Multiome | 856 | |
| chr5:140,673,200–140,673,759 | 17.8 kb | Distal (>10kb) Multiome | 144 | |
| chr5:140,691,053–140,691,933 | 158 bp | At TSS Multiome | 924 | |
| chr5:140,696,939–140,697,394 | 5.6 kb | Proximal (<10kb) | 21 | |
| chr5:140,699,981–140,700,742 | 9.0 kb | Proximal (<10kb) Multiome | 607 | |
| chr5:140,710,433–140,711,671 | 19.7 kb | Distal (>10kb) Multiome | 856 | |
| chr5:140,718,340–140,718,978 | 27.3 kb | Distal (>10kb) Multiome | 763 | |
| chr5:140,725,570–140,726,438 | 34.6 kb | Distal (>10kb) Multiome | 719 | |
| chr5:140,925,961–140,927,592 | 235.2 kb | Distal (>10kb) Multiome | 419 | |
| chr5:140,965,919–140,967,712 | 275.3 kb | Distal (>10kb) Multiome | 368 |
Genomic view of the HARS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.