Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015]
Transcription factors with Perturb-seq knockdown data for H3-3B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = H3-3B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of H3-3B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:75,481,081–75,481,680 | 298.4 kb | Distal (>10kb) Multiome | 64 | |
| chr17:75,514,425–75,517,171 | 264.1 kb | Distal (>10kb) Multiome | 1158 | |
| chr17:75,524,764–75,527,160 | 254.3 kb | Distal (>10kb) Multiome | 951 | |
| chr17:75,549,465–75,550,388 | 229.8 kb | Distal (>10kb) Multiome | 602 | |
| chr17:75,587,697–75,590,735 | 190.4 kb | Distal (>10kb) Multiome | 744 | |
| chr17:75,633,694–75,634,328 | 145.7 kb | Distal (>10kb) Multiome | 559 | |
| chr17:75,666,763–75,667,767 | 112.5 kb | Distal (>10kb) Multiome | 783 | |
| chr17:75,720,933–75,721,693 | 58.4 kb | Distal (>10kb) Multiome | 509 | |
| chr17:75,753,534–75,754,285 | 25.9 kb | Distal (>10kb) Multiome | 699 | |
| chr17:75,764,621–75,765,674 | 14.6 kb | Distal (>10kb) Multiome | 769 | |
| chr17:75,773,398–75,774,736 | 5.0 kb | Proximal (<10kb) | 179 | |
| chr17:75,778,786–75,780,191 | 107 bp | At TSS Multiome | 1118 | |
| chr17:75,784,060–75,785,640 | 5.1 kb | Proximal (<10kb) Multiome | 1176 | |
| chr17:75,827,420–75,828,293 | 48.0 kb | Distal (>10kb) Multiome | 404 | |
| chr17:75,830,158–75,830,695 | 50.6 kb | Distal (>10kb) Multiome | 190 | |
| chr17:75,832,912–75,833,396 | 53.4 kb | Distal (>10kb) Multiome | 104 | |
| chr17:75,845,103–75,845,662 | 65.6 kb | Distal (>10kb) Multiome | 703 | |
| chr17:75,854,955–75,856,363 | 75.7 kb | Distal (>10kb) Multiome | 1038 | |
| chr17:75,877,486–75,878,900 | 98.3 kb | Distal (>10kb) Multiome | 884 | |
| chr17:75,896,392–75,897,754 | 117.2 kb | Distal (>10kb) Multiome | 831 | |
| chr17:75,904,371–75,905,532 | 125.3 kb | Distal (>10kb) Multiome | 1045 | |
| chr17:75,940,734–75,941,471 | 161.3 kb | Distal (>10kb) Multiome | 982 | |
| chr17:75,978,646–75,980,102 | 199.6 kb | Distal (>10kb) Multiome | 1090 | |
| chr17:75,983,292–75,984,226 | 204.0 kb | Distal (>10kb) Multiome | 236 | |
| chr17:76,014,120–76,014,727 | 234.6 kb | Distal (>10kb) Multiome | 489 | |
| chr17:76,029,331–76,030,158 | 249.9 kb | Distal (>10kb) Multiome | 722 | |
| chr17:76,072,173–76,072,853 | 292.8 kb | Distal (>10kb) Multiome | 893 | |
| chr17:76,074,414–76,075,796 | 294.9 kb | Distal (>10kb) Multiome | 280 |
Genomic view of the H3-3B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.