Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]
Transcription factors with Perturb-seq knockdown data for H1-0. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = H1-0 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of H1-0, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:37,518,831–37,519,884 | 285.7 kb | Distal (>10kb) Multiome | 548 | |
| chr22:37,545,569–37,546,885 | 258.9 kb | Distal (>10kb) Multiome | 682 | |
| chr22:37,559,893–37,560,965 | 244.9 kb | Distal (>10kb) Multiome | 589 | |
| chr22:37,569,013–37,570,549 | 235.7 kb | Distal (>10kb) Multiome | 695 | |
| chr22:37,608,044–37,609,455 | 196.5 kb | Distal (>10kb) Multiome | 920 | |
| chr22:37,639,157–37,639,951 | 165.7 kb | Distal (>10kb) Multiome | 427 | |
| chr22:37,658,172–37,659,508 | 146.6 kb | Distal (>10kb) Multiome | 722 | |
| chr22:37,674,916–37,675,641 | 129.8 kb | Distal (>10kb) Multiome | 607 | |
| chr22:37,676,590–37,677,674 | 128.0 kb | Distal (>10kb) Multiome | 655 | |
| chr22:37,686,104–37,686,784 | 118.8 kb | Distal (>10kb) Multiome | 866 | |
| chr22:37,696,496–37,697,685 | 108.3 kb | Distal (>10kb) Multiome | 428 | |
| chr22:37,745,641–37,746,995 | 59.1 kb | Distal (>10kb) Multiome | 627 | |
| chr22:37,802,124–37,802,989 | 2.8 kb | Proximal (<10kb) Multiome | 603 | |
| chr22:37,803,341–37,803,517 | 1.7 kb | Proximal (<10kb) | 376 | |
| chr22:37,804,679–37,805,768 | 5 bp | At TSS Multiome | 763 | |
| chr22:37,807,436–37,808,267 | 2.6 kb | Proximal (<10kb) Multiome | 766 | |
| chr22:37,817,672–37,819,114 | 12.7 kb | Distal (>10kb) Multiome | 713 | |
| chr22:37,824,524–37,825,593 | 19.7 kb | Distal (>10kb) Multiome | 173 | |
| chr22:37,843,748–37,844,743 | 39.2 kb | Distal (>10kb) Multiome | 798 | |
| chr22:37,849,215–37,849,709 | 44.2 kb | Distal (>10kb) Multiome | 753 | |
| chr22:37,905,849–37,906,846 | 101.0 kb | Distal (>10kb) Multiome | 552 | |
| chr22:37,933,777–37,934,256 | 128.9 kb | Distal (>10kb) Multiome | 679 | |
| chr22:37,953,153–37,954,120 | 148.4 kb | Distal (>10kb) Multiome | 617 | |
| chr22:37,983,021–37,985,110 | 179.2 kb | Distal (>10kb) Multiome | 228 | |
| chr22:38,052,312–38,052,803 | 247.3 kb | Distal (>10kb) Multiome | 529 | |
| chr22:38,057,008–38,057,702 | 252.1 kb | Distal (>10kb) Multiome | 656 | |
| chr22:38,080,912–38,081,618 | 275.9 kb | Distal (>10kb) Multiome | 175 | |
| chr22:38,097,697–38,098,236 | 292.7 kb | Distal (>10kb) Multiome | 186 |
Genomic view of the H1-0 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.