GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GTPBP4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GTPBP4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GTPBP4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:688,745–690,650 | 298.6 kb | Distal (>10kb) Multiome | 470 | |
| chr10:692,735–693,228 | 295.4 kb | Distal (>10kb) Multiome | 89 | |
| chr10:930,617–932,295 | 56.6 kb | Distal (>10kb) Multiome | 658 | |
| chr10:973,560–974,740 | 14.4 kb | Distal (>10kb) Multiome | 415 | |
| chr10:987,707–988,902 | 79 bp | At TSS Multiome | 842 | |
| chr10:1,048,018–1,050,104 | 60.7 kb | Distal (>10kb) Multiome | 1021 | |
| chr10:1,056,026–1,057,235 | 68.3 kb | Distal (>10kb) Multiome | 863 |
Genomic view of the GTPBP4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.