Transcription factors with Perturb-seq knockdown data for GOLPH3-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GOLPH3-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GOLPH3-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:32,173,339–32,175,075 | 327 bp | At TSS Multiome | 1090 | |
| chr5:32,287,695–32,288,492 | 114.2 kb | Distal (>10kb) Multiome | 161 | |
| chr5:32,312,016–32,313,764 | 139.1 kb | Distal (>10kb) Multiome | 877 | |
| chr5:32,443,333–32,445,482 | 270.8 kb | Distal (>10kb) Multiome | 960 | |
| chr5:32,463,190–32,463,817 | 289.6 kb | Distal (>10kb) Multiome | 67 | |
| chr5:32,474,004–32,474,924 | 300.4 kb | Distal (>10kb) Multiome | 130 |
Genomic view of the GOLPH3-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.