The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Transcription factors with Perturb-seq knockdown data for GNAI2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GNAI2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GNAI2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:49,939,635–49,940,747 | 296.1 kb | Distal (>10kb) Multiome | 949 | |
| chr3:50,088,748–50,089,596 | 147.3 kb | Distal (>10kb) Multiome | 677 | |
| chr3:50,121,279–50,122,037 | 114.5 kb | Distal (>10kb) Multiome | 364 | |
| chr3:50,138,509–50,140,035 | 96.9 kb | Distal (>10kb) Multiome | 198 | |
| chr3:50,154,437–50,155,741 | 81.2 kb | Distal (>10kb) Multiome | 450 | |
| chr3:50,204,913–50,205,545 | 31.0 kb | Distal (>10kb) Multiome | 367 | |
| chr3:50,205,645–50,206,357 | 30.0 kb | Distal (>10kb) Multiome | 347 | |
| chr3:50,226,120–50,228,328 | 10.0 kb | Proximal (<10kb) Multiome | 915 | |
| chr3:50,235,551–50,236,824 | 182 bp | At TSS Multiome | 632 | |
| chr3:50,237,081–50,237,268 | 878 bp | At TSS | 140 | |
| chr3:50,237,779–50,238,432 | 1.6 kb | Proximal (<10kb) | 342 | |
| chr3:50,240,985–50,241,805 | 5.1 kb | Proximal (<10kb) Multiome | 137 | |
| chr3:50,245,721–50,247,161 | 10.1 kb | Distal (>10kb) Multiome | 671 | |
| chr3:50,259,568–50,260,922 | 24.0 kb | Distal (>10kb) Multiome HiCAR | 869 | |
| chr3:50,264,309–50,265,446 | 28.6 kb | Distal (>10kb) Multiome HiCAR | 568 | |
| chr3:50,275,163–50,276,639 | 40.3 kb | Distal (>10kb) Multiome HiCAR | 151 | |
| chr3:50,291,628–50,292,796 | 56.3 kb | Distal (>10kb) Multiome | 855 | |
| chr3:50,298,495–50,300,110 | 63.3 kb | Distal (>10kb) Multiome | 872 | |
| chr3:50,303,363–50,303,840 | 67.4 kb | Distal (>10kb) Multiome | 484 | |
| chr3:50,320,603–50,322,739 | 85.4 kb | Distal (>10kb) Multiome | 816 | |
| chr3:50,324,456–50,325,020 | 88.5 kb | Distal (>10kb) Multiome | 392 | |
| chr3:50,327,933–50,328,581 | 92.1 kb | Distal (>10kb) Multiome | 697 | |
| chr3:50,336,856–50,338,227 | 101.4 kb | Distal (>10kb) Multiome | 952 | |
| chr3:50,340,624–50,341,322 | 104.7 kb | Distal (>10kb) Multiome | 345 | |
| chr3:50,345,443–50,346,042 | 109.5 kb | Distal (>10kb) Multiome | 209 | |
| chr3:50,350,496–50,351,298 | 114.7 kb | Distal (>10kb) Multiome | 902 | |
| chr3:50,358,889–50,360,012 | 123.4 kb | Distal (>10kb) Multiome | 796 | |
| chr3:50,364,552–50,365,867 | 129.1 kb | Distal (>10kb) Multiome | 331 | |
| chr3:50,435,932–50,436,533 | 200.0 kb | Distal (>10kb) Multiome | 248 | |
| chr3:50,502,505–50,504,160 | 267.5 kb | Distal (>10kb) Multiome | 312 |
Genomic view of the GNAI2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.