In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GMPS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GMPS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GMPS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:155,362,992–155,363,934 | 507.3 kb | Distal (>10kb) Multiome HiCAR | 308 | |
| chr3:155,744,096–155,745,472 | 126.4 kb | Distal (>10kb) Multiome | 726 | |
| chr3:155,745,739–155,746,446 | 124.4 kb | Distal (>10kb) Multiome | 309 | |
| chr3:155,805,529–155,806,648 | 64.4 kb | Distal (>10kb) Multiome | 810 | |
| chr3:155,853,753–155,855,235 | 16.0 kb | Distal (>10kb) Multiome | 1097 | |
| chr3:155,862,681–155,862,854 | 7.8 kb | Proximal (<10kb) | 104 | |
| chr3:155,869,838–155,871,872 | 150 bp | At TSS Multiome | 931 | |
| chr3:155,980,533–155,981,958 | 110.4 kb | Distal (>10kb) Multiome | 149 |
Genomic view of the GMPS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.