Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of DNA-templated transcription. Predicted to be located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for GLI4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GLI4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GLI4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:143,266,869–143,267,949 | 48 bp | At TSS Multiome | 733 | |
| chr8:143,290,813–143,291,709 | 23.9 kb | Distal (>10kb) Multiome | 909 | |
| chr8:143,334,216–143,335,267 | 67.4 kb | Distal (>10kb) Multiome | 688 | |
| chr8:143,368,147–143,369,893 | 101.3 kb | Distal (>10kb) Multiome | 698 | |
| chr8:143,407,212–143,408,612 | 140.7 kb | Distal (>10kb) Multiome | 447 | |
| chr8:143,428,968–143,431,754 | 163.5 kb | Distal (>10kb) Multiome | 743 | |
| chr8:143,540,714–143,542,061 | 274.1 kb | Distal (>10kb) Multiome | 849 | |
| chr8:143,553,122–143,554,177 | 286.3 kb | Distal (>10kb) Multiome | 775 | |
| chr8:143,558,225–143,558,936 | 291.1 kb | Distal (>10kb) Multiome | 600 |
Genomic view of the GLI4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.