This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]
Transcription factors with Perturb-seq knockdown data for GIT2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GIT2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GIT2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:109,697,996–109,698,635 | 298.1 kb | Distal (>10kb) Multiome | 115 | |
| chr12:109,713,288–109,715,156 | 282.6 kb | Distal (>10kb) Multiome | 793 | |
| chr12:109,717,933–109,718,896 | 277.8 kb | Distal (>10kb) Multiome | 199 | |
| chr12:109,737,442–109,738,212 | 258.5 kb | Distal (>10kb) Multiome | 231 | |
| chr12:109,739,699–109,741,057 | 256.2 kb | Distal (>10kb) Multiome | 245 | |
| chr12:109,833,102–109,833,778 | 162.9 kb | Distal (>10kb) Multiome | 217 | |
| chr12:109,845,155–109,845,762 | 150.9 kb | Distal (>10kb) Multiome | 618 | |
| chr12:109,880,019–109,881,173 | 115.8 kb | Distal (>10kb) Multiome | 576 | |
| chr12:109,900,106–109,900,747 | 96.0 kb | Distal (>10kb) Multiome | 818 | |
| chr12:109,995,726–109,996,660 | 84 bp | At TSS Multiome | 811 | |
| chr12:109,998,999–109,999,838 | 2.9 kb | Proximal (<10kb) Multiome | 883 | |
| chr12:110,000,274–110,000,558 | 3.9 kb | Proximal (<10kb) | 111 | |
| chr12:110,048,083–110,048,797 | 52.2 kb | Distal (>10kb) Multiome HiCAR | 921 | |
| chr12:110,107,883–110,108,523 | 111.8 kb | Distal (>10kb) Multiome | 171 | |
| chr12:110,123,690–110,124,996 | 128.0 kb | Distal (>10kb) Multiome | 704 | |
| chr12:110,223,831–110,224,526 | 227.6 kb | Distal (>10kb) Multiome | 199 | |
| chr12:110,280,425–110,282,158 | 284.8 kb | Distal (>10kb) Multiome | 888 |
Genomic view of the GIT2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.