The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611), Psf1, Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for GINS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GINS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GINS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:25,195,309–25,197,066 | 212.0 kb | Distal (>10kb) Multiome | 970 | |
| chr20:25,247,007–25,249,001 | 159.6 kb | Distal (>10kb) Multiome | 1035 | |
| chr20:25,389,726–25,391,241 | 16.8 kb | Distal (>10kb) Multiome | 1021 | |
| chr20:25,391,727–25,391,912 | 720 bp | At TSS | 7 | |
| chr20:25,394,116–25,394,562 | 3.1 kb | Proximal (<10kb) | 29 | |
| chr20:25,399,224–25,399,466 | 8.2 kb | Proximal (<10kb) | 142 | |
| chr20:25,407,111–25,408,013 | 15 bp | At TSS Multiome | 1021 | |
| chr20:25,550,130–25,551,344 | 143.2 kb | Distal (>10kb) Multiome | 207 | |
| chr20:25,584,579–25,586,173 | 177.8 kb | Distal (>10kb) Multiome | 890 | |
| chr20:25,622,823–25,624,816 | 216.3 kb | Distal (>10kb) Multiome | 1127 | |
| chr20:25,626,372–25,627,565 | 219.2 kb | Distal (>10kb) Multiome | 102 | |
| chr20:25,695,981–25,697,222 | 289.1 kb | Distal (>10kb) Multiome | 1015 |
Genomic view of the GINS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.