The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GID4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GID4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GID4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:17,751,962–17,752,874 | 287.1 kb | Distal (>10kb) Multiome | 640 | |
| chr17:17,780,889–17,783,985 | 258.3 kb | Distal (>10kb) Multiome | 655 | |
| chr17:17,792,432–17,793,087 | 246.6 kb | Distal (>10kb) Multiome | 135 | |
| chr17:17,809,458–17,811,066 | 229.4 kb | Distal (>10kb) Multiome | 371 | |
| chr17:17,811,471–17,812,921 | 227.0 kb | Distal (>10kb) Multiome | 664 | |
| chr17:17,823,156–17,824,542 | 215.6 kb | Distal (>10kb) Multiome | 743 | |
| chr17:17,835,765–17,838,695 | 203.0 kb | Distal (>10kb) Multiome | 1030 | |
| chr17:17,839,788–17,841,046 | 199.2 kb | Distal (>10kb) Multiome | 927 | |
| chr17:17,921,363–17,921,988 | 117.7 kb | Distal (>10kb) Multiome | 341 | |
| chr17:17,943,271–17,943,954 | 95.8 kb | Distal (>10kb) Multiome | 143 | |
| chr17:17,971,984–17,973,164 | 66.8 kb | Distal (>10kb) Multiome | 716 | |
| chr17:18,033,570–18,033,778 | 5.6 kb | Proximal (<10kb) | 291 | |
| chr17:18,035,659–18,036,594 | 3.4 kb | Proximal (<10kb) Multiome | 186 | |
| chr17:18,038,656–18,040,279 | 93 bp | At TSS Multiome | 839 | |
| chr17:18,153,365–18,154,352 | 114.5 kb | Distal (>10kb) Multiome | 244 | |
| chr17:18,157,790–18,158,725 | 118.8 kb | Distal (>10kb) Multiome | 292 | |
| chr17:18,182,585–18,185,065 | 144.1 kb | Distal (>10kb) Multiome | 1051 | |
| chr17:18,224,148–18,226,173 | 185.3 kb | Distal (>10kb) Multiome | 680 | |
| chr17:18,246,777–18,247,912 | 207.9 kb | Distal (>10kb) Multiome | 314 | |
| chr17:18,257,865–18,259,083 | 219.3 kb | Distal (>10kb) Multiome | 684 | |
| chr17:18,260,145–18,261,582 | 221.3 kb | Distal (>10kb) Multiome | 778 | |
| chr17:18,314,513–18,315,715 | 275.7 kb | Distal (>10kb) Multiome | 865 |
Genomic view of the GID4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.