The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GEMIN4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GEMIN4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GEMIN4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:714,554–715,298 | 37.3 kb | Distal (>10kb) Multiome | 798 | |
| chr17:731,870–733,144 | 19.8 kb | Distal (>10kb) Multiome | 602 | |
| chr17:751,539–752,928 | 140 bp | At TSS Multiome | 891 | |
| chr17:781,922–782,799 | 30.1 kb | Distal (>10kb) Multiome | 914 | |
| chr17:875,256–875,758 | 123.3 kb | Distal (>10kb) Multiome | 42 | |
| chr17:927,652–928,423 | 175.7 kb | Distal (>10kb) Multiome | 315 | |
| chr17:933,255–933,971 | 181.4 kb | Distal (>10kb) Multiome | 400 | |
| chr17:951,413–952,178 | 199.6 kb | Distal (>10kb) Multiome | 385 | |
| chr17:964,907–965,931 | 213.4 kb | Distal (>10kb) Multiome | 256 | |
| chr17:978,189–980,530 | 227.7 kb | Distal (>10kb) Multiome HiCAR | 639 | |
| chr17:996,479–997,532 | 244.7 kb | Distal (>10kb) Multiome HiCAR | 994 | |
| chr17:1,031,608–1,032,193 | 279.7 kb | Distal (>10kb) Multiome | 54 |
Genomic view of the GEMIN4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.